The paper presents the study of the chemical composition of nicotine by Fourier transform infrared spectroscopy (FTIR). FTIR analysis allowed the identification of functional groups characteristic of the molecule, such as amine groups and C–H bonds of the pyridine and pyrrolidine rings. The results confirmed the molecular structure of nicotine and highlighted the potential of the FTIR method for the rapid identification and characterization of this compound. The study highlights the applicability of FTIR in organic chemistry and in the analysis of bioactive compounds, providing an efficient and non-invasive method for the detection of nicotine in various matrices.