This study analyzed the factors militating against catfish fingerlings production in Anambra State. The study focused on the factors affecting the profitability of fingerlings production and the constraints faced by the producers. A multistage sampling technique was employed in the selection of location and respondents. The data were collected from 100 catfish fingerlings producers. The primary instrument used for data collected was structured questionnaire. Data obtained was analyzed by descriptive statistics and regression analysis. The analysis reveals that age (0.122, p = 0.037), years of experience (0.155, p = 0.000), stocking density (0.200, p = 0.000), and water temperature (0.130, p = 0.003) significantly impact the profitability of catfish fingerlings production. In contrast, costs related to transportation (-0.110, p = 0.022) and communication have a negative effect, while other factors like breeding weight and cost of feeding are not statistically significant. The major constraints include the scarcity of fingerlings and inadequate capital. The study concludes that while catfish farming is profitable, addressing supply and financial constraints is crucial for enhancing production and profitability. Recommendations include improving supply chains, increasing financial support, and investing in training and infrastructure.